Plasmid_Backbone

Part:BBa_M36099

Designed by: Joseph Shih   Group: Stanford BIOE44 - S11   (2013-04-23)


E. coli sensor test rig

E. coli test vector for sensors. Has a Gemini protein coding sequence which produces a LacZalpha-GFP fusion protein. Comes with high copy number origin and ampicillin resistance; can replace with low copy number origin and kanamycin resistance if ordering from DNA 2.0.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2342
    Illegal XbaI site found at 3188
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2342
    Illegal XbaI site found at 3188
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2342
    Illegal XbaI site found at 3188
    Illegal AgeI site found at 2605
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
//plasmidbackbone
Parameters
None